How To Make Liquid Culture From A Spore Syringe. Mushrooms, we provide mushroom grow kits for beginners. Liquid culture is a sterile mixture of water and one of a few specific sugars.
Taking it easy with the grow kits without mycelium? Scrape your spores with your needle attached to your syringe and slowly release water over the spores and into the glass. Get your spore print and clean glass and get it setup in your still air box or in front of your laminar flow hood.
Ensure You Refrigerate If You Intend To Store Them For The Long Term.
10 ounce jar of liquid culture solution, pressure sterilized and. Mushroom spores can last for years. Midwest's premium liquid culture kit saves you time and money on any larger growing project.
Half Fill Each Mason Jar With Liquid Culture Broth.
Let this grow out and then when it is fully grown out take to still air box and using sterile methods place a colonized popcorn directly onto agar. Soon you will see myc forming. Remove the shot glass/small dish from the pressure cooker.
However, You Must Take Senescence Into Consideration (So Use A Master Jar).
Inside most spore syringes are thousands/millions of genetic lineages. What is the shelf life of a liquid spore culture? With your tweezers, take the spore print from its storage and hold it over the opening of the shot glass/small dish.
Bore A Small Hole Big Enough For A Syringe Needle In The Lid/Screw Cap Of Your Jar.
(half pints work best for this). A spore syringe is a solution of fungal spores floating in sterile water. Liquid culture can be transferred from a syringe to agar, liquid media (to make more liquid culture), grains, and bulk substrates.
In Case You Want To Make Liquid Culture From The Mushroom Spores, Use The Spore Vial Or Print Aside From The Spore Syringe.
Turn one spore syringe into 10 or more! Now inject 1cc and shake. Stick this in the port.